How to build a 2d and 3d aerial multispectral map?—all steps deeply explained

UIDB/04111/2020 PCIF/SSI/0102/2017 IF/00325/2015 UIDB/00066/2020The increased development of camera resolution, processing power, and aerial platforms helped to create more cost-efficient approaches to capture and generate point clouds to assist in scientific fields. The continuous development of methods to produce three-dimensional models based on two-dimensional images such as Structure from Motion (SfM) and Multi-View Stereopsis (MVS) allowed to improve the resolution of the produced models by a significant amount. By taking inspiration from the free and accessible workflow made available by OpenDroneMap, a detailed analysis of the processes is displayed in this paper. As of the writing of this paper, no literature was found that described in detail the necessary steps and processes that would allow the creation of digital models in two or three dimensions based on aerial images. With this, and based on the workflow of OpenDroneMap, a detailed study was performed. The digital model reconstruction process takes the initial aerial images obtained from the field survey and passes them through a series of stages. From each stage, a product is acquired and used for the following stage, for example, at the end of the initial stage a sparse reconstruction is produced, obtained by extracting features of the images and matching them, which is used in the following step, to increase its resolution. Additionally, from the analysis of the workflow, adaptations were made to the standard workflow in order to increase the compatibility of the developed system to different types of image sets. Particularly, adaptations focused on thermal imagery were made. Due to the low presence of strong features and therefore difficulty to match features across thermal images, a modification was implemented, so thermal models could be produced alongside the already implemented processes for multispectral and RGB image sets.publishersversionpublishe

Inarticulate devices: Critical encounters with network technology in research through design

Research through design (RTD) is commonly conceived as a material and discursive practice of articulating knowledge. This paper contributes to the understanding of RTD as a form of critical inquiry by considering how inarticulacy can also be a productive element of this process. We present two reflective accounts of critically-engaged RTD practices in which our attempts to articulate concerns or questions were met with resistance from technology that was both the subject and medium of our investigation. We argue that encountering inarticulacy is not a failure of RTD but instead points to how material exploration can sensitise us to how network technology resists articulating certain values or concerns. Encountering inarticulacy led us to formulate new problems and new lines of inquiry. We conclude by suggesting that the central role given to ambiguity in RTD prepares us to witness and respond to inarticulacy in our practices, design outcomes and critical understandings

S-Glutathionylation at Cys328 and Cys542 Impairs STAT3 Phosphorylation.

STAT3 is a latent transcription factor that promotes cell survival and proliferation and is often constitutively active in cancers. Although many reports provide evidence that STAT3 is a direct target of oxidative stress, its redox regulation is poorly understood. Under oxidative conditions STAT3 activity can be modulated by S-glutathionylation, a reversible redox modification of cysteine residues. This suggests the possible cross-talk between phosphorylation and glutathionylation and points out that STAT3 is susceptible to redox regulation. Recently, we reported that decreasing the GSH content in different cell lines induces inhibition of STAT3 activity through the reversible oxidation of thiol groups. In the present work, we demonstrate that GSH/diamide treatment induces S-glutathionylation of STAT3 in the recombinant purified form. This effect was completely reversed by treatment with the reducing agent dithiothreitol, indicating that S-glutathionylation of STAT3 was related to formation of protein-mixed disulfides. Moreover, addition of the bulky negatively charged GSH moiety impairs JAK2-mediated STAT3 phosphorylation, very likely interfering with tyrosine accessibility and thus affecting protein structure and function. Mass mapping analysis identifies two glutathionylated cysteine residues, Cys328 and Cys542, within the DNA-binding domain and the linker domain, respectively. Site direct mutagenesis and in vitro kinase assay confirm the importance of both cysteine residues in the complex redox regulatory mechanism of STAT3. Cells expressing mutant were resistant in this regard. The data presented herein confirmed the occurrence of a redox-dependent regulation of STAT3, identified the more redox-sensitive cysteines within STAT3 structure, and may have important implications for development of new drugs

OpenDroneMap: The Missing Guide. A Practical Guide To Drone Mapping Using Free and Open Source Software

https://www.ester.ee/record=b5451442*es

OpenDroneMap/ODM: 3.2.1

What's Changed Always use matcher-neighbors if less than 2 pictures by @pierotofy in https://github.com/OpenDroneMap/ODM/pull/1694 Fix minimum number of pictures for matcher neighbors by @pierotofy in https://github.com/OpenDroneMap/ODM/pull/1695 More memory efficient find_features_homography by @pierotofy in https://github.com/OpenDroneMap/ODM/pull/1696 Compress GCP data before VLR inclusion by @pierotofy in https://github.com/OpenDroneMap/ODM/pull/1697 Full Changelog: https://github.com/OpenDroneMap/ODM/compare/v3.2.0...v3.2.